Genetics and Molecular Probes
HAB scientists use two methods of DNA analysis to study HAB and non-HAB species. The two methods, genetic analysis and molecular probe analysis, help distinguish similar species.
The HAB group at FWRI studies the DNA of HAB species using two different research methods, genetic analysis and molecular probe analysis. Genetic analysis uses polymerase chain reaction and DNA sequencing to study the evolutionary relationships between HAB and non-HAB species and to help identify similar species. Molecular probe analysis uses fluorescent probes to target the DNA sequence specific to a single algal species, such as Karenia brevis, the Florida red tide organism. A species-specific probe decreases the potential for misidentification in similar species. The HAB group is currently adapting genetic probes for incorporation into an automated, near-real-time monitoring system to detect low levels of toxic algal species in offshore areas. Automatic detection of low levels of HAB species such as K. brevis will provide early warning of a potential offshore bloom and, thus, help monitor the development and movement of blooms.
